GFP-specific CD8 T cells enable targeted cell depletion and visualization of T-cell interactions.

ABSTRACT: There are numerous cell types with scarcely understood functions, whose interactions with the immune system are not well characterized. To facilitate their study, we generated a mouse bearing enhanced green fluorescent protein (EGFP)-specific CD8⁺ T cells. Transfer of the T cells into EGFP reporter animals can be used to kill EGFP-expressing cells, allowing selective depletion of desired cell types, or to interrogate T-cell interactions with specific populations. Using this system, we eliminate a rare EGFP-expressing cell type in the heart and demonstrate its role in cardiac function. We also show that naive T cells are recruited into the mouse brain by antigen-expressing microglia, providing evidence of an immune surveillance pathway in the central nervous system. The just EGFP

SUBMITTER: Agudo J 

PROVIDER: S-EPMC4675673 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature