Captured metagenomics: large-scale targeting of genes based on 'sequence capture' reveals functional diversity in soils.

ABSTRACT: Microbial enzyme diversity is a key to understand many ecosystem processes. Whole metagenome sequencing (WMG) obtains information on functional genes, but it is costly and inefficient due to large amount of sequencing that is required. In this study, we have applied a captured metagenomics technique for functional genes in soil microorganisms, as an alternative to WMG. Large-scale targeting of functional genes, coding for enzymes related to organic matter degradation, was applied to two agricultural soil communities through captured metagenomics. Captured metagenomics uses custom-designed, hybridization-based oligonucleotide probes that enrich functional genes of interest in metagenomic libraries where only probe-bound DNA fragments are sequenced. The captured metagenomes were highly enric

SUBMITTER: Manoharan L 

PROVIDER: S-EPMC4675713 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature