Pressure and Temperature Effects on the Activity and Structure of the Catalytic Domain of Human MT1-MMP.

ABSTRACT: Membrane type 1-matrix metalloproteinase (MT1-MMP or MMP-14) is a zinc-transmembrane metalloprotease involved in the degradation of extracellular matrix and tumor invasion. While changes in solvation of MT1-MMP have been recently studied, little is known about the structural and energetic changes associated with MT1-MMP while interacting with substrates. Steady-state kinetic and thermodynamic data (including activation energies and activation volumes) were measured over a wide range of temperatures and pressures by means of a stopped-flow fluorescence technique. Complementary temperature- and pressure-dependent Fourier-transform infrared measurements provided corresponding structural information of the protein. MT1-MMP is stable and active over a wide range of temperatures (10-55 °C). A sm

SUBMITTER: Decaneto E 

PROVIDER: S-EPMC4675863 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature