Oxidative Stress Alters the Morphology and Toxicity of Aortic Medial Amyloid.

ABSTRACT: The aggregation and fibril deposition of amyloid proteins have been implicated in a range of neurodegenerative and vascular diseases, and yet the underlying molecular mechanisms are poorly understood. Here, we use a combination of cell-based assays, biophysical analysis, and atomic force microscopy to investigate the potential involvement of oxidative stress in aortic medial amyloid (AMA) pathogenesis and deposition. We show that medin, the main constituent of AMA, can induce an environment rich in oxidative species, increasing superoxide and reducing bioavailable nitric oxide in human cells. We investigate the role that this oxidative environment may play in altering the aggregation process of medin and identify potential posttranslational modification sites where site-specific modificati

SUBMITTER: Davies HA 

PROVIDER: S-EPMC4675884 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature