Redox State of Cytochromes in Frozen Yeast Cells Probed by Resonance Raman Spectroscopy.

ABSTRACT: Cryopreservation is a well-established technique used for the long-term storage of biological materials whose biological activity is effectively stopped under low temperatures (suspended animation). Since most biological methods do not work in a low-temperature frozen environment, the mechanism and details of the depression of cellular activity in the frozen state remain largely uncharacterized. In this work, we propose, to our knowledge, a new approach to study the downregulation of the redox activity of cytochromes b and c in freezing yeast cells in a contactless, label-free manner. Our approach is based on cytochrome photobleaching effects observed in the resonance Raman spectra of live cells. Photoinduced and native redox reactions that contributed to the photobleaching rate were studi

SUBMITTER: Okotrub KA 

PROVIDER: S-EPMC4675887 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature