Dataset Information

Uncovering MicroRNA Regulatory Hubs that Modulate Plasma Cell Differentiation.

ABSTRACT: Using genome-wide approaches, we studied the microRNA (miRNA) expression profile during human plasma cell (PC) differentiation induced by stimulation of human blood B cells with T follicular helper cell-dependent signals. Combining the profiles of differentially expressed genes in PC differentiation with gene ontology (GO) analysis revealed that a significant group of genes involved in the transcription factor (TF) activity was preferentially changed. We thus focused on studying the effects of differentially expressed miRNAs on several key TFs in PC differentiation. Cohorts of differentially expressed miRNAs cooperating as miRNA hubs were predicted and validated to modulate key TFs, including a down-regulated miRNA hub containing miR-101-3p, -125b-5p, and -223-3p contributing to induction of PRDM1 as well as an up-regulated miRNA hub containing miR-34a-5p, -148a-3p, and -183-5p suppressing BCL6, BACH2, and FOXP1. Induced expression of NF-κB and PRDM1 during PC differentiation controlled the expression of up- and down-regulated miRNA hubs, respectively. Co-expression of miR-101-3p, -125b-5p, and -223-3p in stimulated B cells showed synergistic effects on inhibition of PC formation, which can be rescued by re-introduction of PRDM1. Together, we catalogue the complex roadmap of miRNAs and their functional interplay in collaboratively directing PC differentiation.

SUBMITTER: Tsai DY

PROVIDER: S-EPMC4675970 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature

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Uncovering MicroRNA Regulatory Hubs that Modulate Plasma Cell Differentiation.

Tsai Dong-Yan DY Hung Kuo-Hsuan KH Lin I-Ying IY Su Shin-Tang ST Wu Shih-Ying SY Chung Cheng-Han CH Wang Tong-Cheng TC Li Wen-Hsiung WH Li Wen-Hsiung WH Shih Arthur Chun-Chieh AC Lin Kuo-I KI

Scientific reports 20151211

Using genome-wide approaches, we studied the microRNA (miRNA) expression profile during human plasma cell (PC) differentiation induced by stimulation of human blood B cells with T follicular helper cell-dependent signals. Combining the profiles of differentially expressed genes in PC differentiation with gene ontology (GO) analysis revealed that a significant group of genes involved in the transcription factor (TF) activity was preferentially changed. We thus focused on studying the effects of d ...[more]

PMID: 26655851

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Uncovering MicroRNA Regulatory Hubs that Modulate Plasma Cell Differentiation.

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Uncovering MicroRNA Regulatory Hubs that Modulate Plasma Cell Differentiation.

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