Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells.

ABSTRACT: Chinese hamster ovary (CHO) cells are widely used as cell factories for the production of biopharmaceuticals. In contrast to the highly optimized production processes for monoclonal antibody (mAb)-based biopharmaceuticals, improving productivity of non-mAb therapeutic glycoproteins is more likely to reduce production costs significantly. The aim of this study was to establish a versatile target gene screening platform for improving productivity for primarily non-mAb glycoproteins with complete interchangeability of model proteins and target genes using transient expression. The platform consists of four techniques compatible with 96-well microplates: lipid-based transient transfection, cell cultivation in microplates, cell counting and antibody-independent product titer determination based

SUBMITTER: Hansen HG 

PROVIDER: S-EPMC4676018 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature