The Murine Lung Microbiome Changes During Lung Inflammation and Intranasal Vancomycin Treatment.

ABSTRACT: Most microbiome research related to airway diseases has focused on the gut microbiome. This is despite advances in culture independent microbial identification techniques revealing that even healthy lungs possess a unique dynamic microbiome. This conceptual change raises the question; if lung diseases could be causally linked to local dysbiosis of the local lung microbiota. Here, we manipulate the murine lung and gut microbiome, in order to show that the lung microbiota can be changed experimentally. We have used four different approaches: lung inflammation by exposure to carbon nano-tube particles, oral probiotics and oral or intranasal exposure to the antibiotic vancomycin. Bacterial DNA was extracted from broncho-alveolar and nasal lavage fluids, caecum samples and compared by DGGE. Our

SUBMITTER: Barfod KK 

PROVIDER: S-EPMC4676059 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature