A dual role of transient receptor potential melastatin 2 channel in cytotoxicity induced by silica nanoparticles.

ABSTRACT: Silica nanoparticles (NPs) have remarkable applications. However, accumulating evidence suggests NPs can cause cellular toxicity by inducing ROS production and increasing intracellular Ca(2+) ([Ca(2+)]i), but the underlying molecular mechanism is largely unknown. Transient receptor potential melastatin 2 (TRPM2) channel is known to be a cellular redox potential sensor that provides an important pathway for increasing the [Ca(2+)]i under oxidative stress. In this study, we examined the role of TRPM2 channel in silica NPs-induced oxidative stress and cell death. By quantitation of cell viability, ROS production, [Ca(2+)]i, and protein identification, we showed that TRPM2 channel is required for ROS production and Ca(2+) increase induced by silica NPs through regulating NADPH oxidase activity

SUBMITTER: Yu P 

PROVIDER: S-EPMC4676061 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature