IS26-Mediated Precise Excision of the IS26-aphA1a Translocatable Unit.

ABSTRACT:

Unlabelled

We recently showed that, in the absence of RecA-dependent homologous recombination, the Tnp26 transposase catalyzes cointegrate formation via a conservative reaction between two preexisting IS26, and this is strongly preferred over replicative transposition to a new site. Here, the reverse reaction was investigated by assaying for precise excision of the central region together with a single IS26 from a compound transposon bounded by IS26. In a recA mutant strain, Tn4352, a kanamycin resistance transposon carrying the aphA1a gene, was stable. However, loss of kanamycin resistance due to precise excision of the translocatable unit (TU) from the closely related Tn4352B, leaving behind the second IS26, occurred at high frequency. Excision occurred when Tn4352B was in either

SUBMITTER: Harmer CJ 

PROVIDER: S-EPMC4676283 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature