Impact of Anaerobiosis on Expression of the Iron-Responsive Fur and RyhB Regulons.

ABSTRACT:

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Iron, a major protein cofactor, is essential for most organisms. Despite the well-known effects of O2 on the oxidation state and solubility of iron, the impact of O2 on cellular iron homeostasis is not well understood. Here we report that in Escherichia coli K-12, the lack of O2 dramatically changes expression of genes controlled by the global regulators of iron homeostasis, the transcription factor Fur and the small RNA RyhB. Using chromatin immunoprecipitation sequencing (ChIP-seq), we found anaerobic conditions promote Fur binding to more locations across the genome. However, by expression profiling, we discovered that the major effect of anaerobiosis was to increase the magnitude of Fur regulation, leading to increased expression of iron storage proteins and decrease

SUBMITTER: Beauchene NA 

PROVIDER: S-EPMC4676285 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature