Contribution of Physical Interactions to Signaling Specificity between a Diguanylate Cyclase and Its Effector.

ABSTRACT:

Unlabelled

Cyclic diguanylate (c-di-GMP) is a bacterial second messenger that controls multiple cellular processes. c-di-GMP networks have up to dozens of diguanylate cyclases (DGCs) that synthesize c-di-GMP along with many c-di-GMP-responsive target proteins that can bind and respond to this signal. For such networks to have order, a mechanism(s) likely exists that allow DGCs to specifically signal their targets, and it has been suggested that physical interactions might provide such specificity. Our results show a DGC from Pseudomonas fluorescens physically interacting with its target protein at a conserved interface, and this interface can be predictive of DGC-target protein interactions. Furthermore, we demonstrate that physical interaction is necessary for the DGC to maximally

SUBMITTER: Dahlstrom KM 

PROVIDER: S-EPMC4676286 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature