Genetic and epigenetic variation among inbred mouse littermates: identification of inter-individual differentially methylated regions.

ABSTRACT:

Background

Phenotypic variability among inbred littermates reared in controlled environments remains poorly understood. Metastable epialleles refer to loci that intrinsically behave in this way and a few examples have been described. They display differential methylation in association with differential expression. For example, inbred mice carrying the agouti viable yellow (A (vy) ) allele show a range of coat colours associated with different DNA methylation states at the locus. The availability of next-generation sequencing, in particular whole genome sequencing of bisulphite converted DNA, allows us, for the first time, to search for metastable epialleles at base pair resolution.

Results

Using whole genome bisulphite sequencing of DNA from the livers of five mice from the

SUBMITTER: Oey H 

PROVIDER: S-EPMC4676890 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature