High-Throughput Non-Contact Vitrification of Cell-Laden Droplets Based on Cell Printing.

ABSTRACT: Cryopreservation is the most promising way for long-term storage of biological samples e.g., single cells and cellular structures. Among various cryopreservation methods, vitrification is advantageous by employing high cooling rate to avoid the formation of harmful ice crystals in cells. Most existing vitrification methods adopt direct contact of cells with liquid nitrogen to obtain high cooling rates, which however causes the potential contamination and difficult cell collection. To address these limitations, we developed a non-contact vitrification device based on an ultra-thin freezing film to achieve high cooling/warming rate and avoid direct contact between cells and liquid nitrogen. A high-throughput cell printer was employed to rapidly generate uniform cell-laden microdroplets into

SUBMITTER: Shi M 

PROVIDER: S-EPMC4677291 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature