Directly visualized glioblastoma-derived extracellular vesicles transfer RNA to microglia/macrophages in the brain.

ABSTRACT:

Background

To understand the ability of gliomas to manipulate their microenvironment, we visualized the transfer of vesicles and the effects of tumor-released extracellular RNA on the phenotype of microglia in culture and in vivo.

Methods

Extracellular vesicles (EVs) released from primary human glioblastoma (GBM) cells were isolated and microRNAs (miRNAs) were analyzed. Primary mouse microglia were exposed to GBM-EVs, and their uptake and effect on proliferation and levels of specific miRNAs, mRNAs, and proteins were analyzed. For in vivo analysis, mouse glioma cells were implanted in the brains of mice, and EV release and uptake by microglia and monocytes/macrophages were monitored by intravital 2-photon microscopy, immunohistochemistry, and fluorescence activated cell sort

SUBMITTER: van der Vos KE 

PROVIDER: S-EPMC4677420 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature