Compartmentalized Toxoplasma EB1 bundles spindle microtubules to secure accurate chromosome segregation.

ABSTRACT: Toxoplasma gondii replicates asexually by a unique internal budding process characterized by interwoven closed mitosis and cytokinesis. Although it is known that the centrosome coordinates these processes, the spatiotemporal organization of mitosis remains poorly defined. Here we demonstrate that centrosome positioning around the nucleus may signal spindle assembly: spindle microtubules (MTs) are first assembled when the centrosome moves to the basal side and become extensively acetylated after the duplicated centrosomes reposition to the apical side. We also tracked the spindle MTs using the MT plus end-binding protein TgEB1. Endowed by a C-terminal NLS, TgEB1 resides in the nucleoplasm in interphase and associates with the spindle MTs during mitosis. TgEB1 also associates with the subpel

SUBMITTER: Chen CT 

PROVIDER: S-EPMC4678015 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature