Vesicular Location and Transport of S100A8 and S100A9 Proteins in Monocytoid Cells.

ABSTRACT: We show here, by using surface biotinylation, followed by Western blotting or surface plasmon resonance analysis, that very low levels of S100A8 and/or S100A9 can be detected on the surface of THP-1 cells or freshly isolated human monocytes. This was supported by immune-electron microscopy where we observed membrane-associated expression of the proteins restricted to small patches. By using confocal microscopy we could determine that S100A8 and S100A9 protein in THP-1 cells or freshly isolated human monocytes was mostly present in vesicular structures. This finding was confirmed using immune-electron microscopy. Subcellular fractionation and confocal microscopy showed that these vesicular structures are mainly early endosomes and endolysosomes. Our subsequent studies showed that accumulati

SUBMITTER: Chakraborty P 

PROVIDER: S-EPMC4678419 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature