Spatially transformed fluorescence image data for ERK-MAPK and selected proteins within human epidermis.

ABSTRACT:

Background

Phosphoprotein signalling pathways have been intensively studied in vitro, yet their role in regulating tissue homeostasis is not fully understood. In the skin, interfollicular keratinocytes differentiate over approximately 2 weeks as they traverse the epidermis. The extracellular signal-regulated kinase (ERK) branch of the mitogen-activated protein kinase (MAPK) pathway has been implicated in this process. Therefore, we examined ERK-MAPK activity within human epidermal keratinocytes in situ.

Findings

We used confocal microscopy and immunofluorescence labelling to measure the relative abundances of Raf-1, MEK1/2 and ERK1/2, and their phosphorylated (active) forms within three human skin samples. Additionally, we measured the abundance of selected proteins thought

SUBMITTER: Cursons J 

PROVIDER: S-EPMC4678632 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature