A novel method for the multiplexed target enrichment of MinION next generation sequencing libraries using PCR-generated baits.

ABSTRACT: The enrichment of targeted regions within complex next generation sequencing libraries commonly uses biotinylated baits to capture the desired sequences. This method results in high read coverage over the targets and their flanking regions. Oxford Nanopore Technologies recently released an USB3.0-interfaced sequencer, the MinION. To date no particular method for enriching MinION libraries has been standardized. Here, using biotinylated PCR-generated baits in a novel approach, we describe a simple and efficient way for multiplexed enrichment of MinION libraries, overcoming technical limitations related with the chemistry of the sequencing-adapters and the length of the DNA fragments. Using Phage Lambda and Escherichia coli as models we selectively enrich for specific targets, significantly

SUBMITTER: Karamitros T 

PROVIDER: S-EPMC4678842 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature