Comprehensive study of liposome-assisted synthesis of membrane proteins using a reconstituted cell-free translation system.

ABSTRACT: Membrane proteins play pivotal roles in cellular processes and are key targets for drug discovery. However, the reliable synthesis and folding of membrane proteins are significant problems that need to be addressed owing to their extremely high hydrophobic properties, which promote irreversible aggregation in hydrophilic conditions. Previous reports have suggested that protein aggregation could be prevented by including exogenous liposomes in cell-free translation processes. Systematic studies that identify which membrane proteins can be rescued from irreversible aggregation during translation by liposomes would be valuable in terms of understanding the effects of liposomes and developing applications for membrane protein engineering in the context of pharmaceutical science and nanodevice

SUBMITTER: Niwa T 

PROVIDER: S-EPMC4678891 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature