Genome Wide Sampling Sequencing for SNP Genotyping: Methods, Challenges and Future Development.

ABSTRACT: Genetic polymorphisms, particularly single nucleotide polymorphisms (SNPs), have been widely used to advance quantitative, functional and evolutionary genomics. Ideally, all genetic variants among individuals should be discovered when next generation sequencing (NGS) technologies and platforms are used for whole genome sequencing or resequencing. In order to improve the cost-effectiveness of the process, however, the research community has mainly focused on developing genome-wide sampling sequencing (GWSS) methods, a collection of reduced genome complexity sequencing, reduced genome representation sequencing and selective genome target sequencing. Here we review the major steps involved in library preparation, the types of adapters used for ligation and the primers designed for amplificati

SUBMITTER: Jiang Z 

PROVIDER: S-EPMC4679402 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature