Circadian rhythm influences genome-wide transcriptional responses to (131)I in a tissue-specific manner in mice.

ABSTRACT:

Background

Circadian variation of gene expression is often neglected when ionizing radiation-induced effects are studied, whether in animal models or in cell culture. This study characterized diurnal variation of genome-wide transcriptional regulation and responses of potential biomarkers and signature genes in normal mouse tissues at 24 h after i.v. administration of (131)I.

Methods

Female BALB/c nude mice were i.v. injected with 90 kBq (131)I at 9:00 a.m., 12:00 p.m., or 3:00 p.m. and killed after 24 h (n = 4/group). Paired control groups were mock-treated (n = 3-4/group). The kidneys, liver, lungs, spleen, and thyroid were excised, snap-frozen, and stored at -80 °C until extraction of total RNA. RNA microarray technology was used for genome-wide expression analysis. Enric

SUBMITTER: Langen B 

PROVIDER: S-EPMC4679710 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature