Heidelberg-mCT-Analyzer: a novel method for standardized microcomputed-tomography-guided evaluation of scaffold properties in bone and tissue research.

ABSTRACT: Bone tissue engineering and bone scaffold development represent two challenging fields in orthopaedic research. Micro-computed tomography (mCT) allows non-invasive measurement of these scaffolds' properties in vivo. However, the lack of standardized mCT analysis protocols and, therefore, the protocols' user-dependency make interpretation of the reported results difficult. To overcome these issues in scaffold research, we introduce the Heidelberg-mCT-Analyzer. For evaluation of our technique, we built 10 bone-inducing scaffolds, which underwent mCT acquisition before ectopic implantation (T0) in mice, and at explantation eight weeks thereafter (T1). The scaffolds' three-dimensional reconstructions were automatically segmented using fuzzy clustering with fully automatic level-setting. The sc

SUBMITTER: Westhauser F 

PROVIDER: S-EPMC4680623 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature