Reproducibility of telomere length assessment: an international collaborative study.

ABSTRACT:

Background

Telomere length is a putative biomarker of ageing, morbidity and mortality. Its application is hampered by lack of widely applicable reference ranges and uncertainty regarding the present limits of measurement reproducibility within and between laboratories.

Methods

We instigated an international collaborative study of telomere length assessment: 10 different laboratories, employing 3 different techniques [Southern blotting, single telomere length analysis (STELA) and real-time quantitative PCR (qPCR)] performed two rounds of fully blinded measurements on 10 human DNA samples per round to enable unbiased assessment of intra- and inter-batch variation between laboratories and techniques.

Results

Absolute results from different laboratories differed widely an

SUBMITTER: Martin-Ruiz CM 

PROVIDER: S-EPMC4681105 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature