Cardiac Disease Status Dictates Functional mRNA Targeting Profiles of Individual MicroRNAs.

ABSTRACT:

Background

MicroRNAs are key players in cardiac stress responses, but the mRNAs, whose abundance and translational potential are primarily affected by changes in cardiac microRNAs, are not well defined. Stimulus-induced, large-scale alterations in the cardiac transcriptome, together with consideration of the law of mass action, further suggest that the mRNAs most substantively targeted by individual microRNAs will vary between unstressed and stressed conditions. To test the hypothesis that microRNA target profiles differ in health and disease, we traced the fate of empirically determined miR-133a and miR-378 targets in mouse hearts undergoing pressure overload hypertrophy.

Methods and results

Ago2 immunoprecipitation with RNA sequencing (RNA-induced silencing complex sequenc

SUBMITTER: Matkovich SJ 

PROVIDER: S-EPMC4681654 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature