Functional tooth restoration utilising split germs through re-regionalisation of the tooth-forming field.

ABSTRACT: The tooth is an ectodermal organ that arises from a tooth germ under the regulation of reciprocal epithelial-mesenchymal interactions. Tooth morphogenesis occurs in the tooth-forming field as a result of reaction-diffusion waves of specific gene expression patterns. Here, we developed a novel mechanical ligation method for splitting tooth germs to artificially regulate the molecules that control tooth morphology. The split tooth germs successfully developed into multiple correct teeth through the re-regionalisation of the tooth-forming field, which is regulated by reaction-diffusion waves in response to mechanical force. Furthermore, split teeth erupted into the oral cavity and restored physiological tooth function, including mastication, periodontal ligament function and responsiveness to

SUBMITTER: Yamamoto N 

PROVIDER: S-EPMC4682098 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature