A novel human leiomyoma tissue derived matrix for cell culture studies.

ABSTRACT:

Background

The composition of the matrix molecules is important in in vitro cell culture experiments of e.g. human cancer invasion and vessel formation. Currently, the mouse Engelbreth-Holm-Swarm (EHS) sarcoma-derived products, such as Matrigel®, are the most commonly used tumor microenvironment (TME) mimicking matrices for experimental studies. However, since Matrigel® is non-human in origin, its molecular composition does not accurately simulate human TME. We have previously described a solid 3D organotypic myoma disc invasion assay, which is derived from human uterus benign leiomyoma tumor. Here, we describe the preparation and analyses of a processed, gelatinous leiomyoma matrix, named Myogel.

Methods

A total protein extract, Myogel, was formulated from myoma. The protei

SUBMITTER: Salo T 

PROVIDER: S-EPMC4682271 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature