Novel MED12 gene somatic mutations in women from the Southern United States with symptomatic uterine fibroids.

ABSTRACT: Although somatic mutations in exon 2 of the mediator complex subunit 12 (MED12) gene have been reported previously in uterine fibroids in women from Finland, South Africa, and North America, the status of these mutations was not reported in the Southern United States women. The aim of this study is to determine the MED12 somatic mutations in uterine fibroids of women from Southern Unites States, which will help to better understand the contribution of MED12 mutations in fibroid tumor biology. Herein, we determined the frequency of MED12 gene exon 2 somatic mutations in 143 fibroid tumors from a total of 135 women from the Southern United States and in 50 samples of the adjacent myometrium using PCR amplification and Sanger sequencing. We observed that the MED12 gene is mutated in 64.33 % (

SUBMITTER: Halder SK 

PROVIDER: S-EPMC4682355 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature