MethGo: a comprehensive tool for analyzing whole-genome bisulfite sequencing data.

ABSTRACT:

Background

DNA methylation is a major epigenetic modification regulating several biological processes. A standard approach to measure DNA methylation is bisulfite sequencing (BS-Seq). BS-Seq couples bisulfite conversion of DNA with next-generation sequencing to profile genome-wide DNA methylation at single base resolution. The analysis of BS-Seq data involves the use of customized aligners for mapping bisulfite converted reads and the bioinformatic pipelines for downstream data analysis.

Results

Here we developed MethGo, a software tool designed for the analysis of data from whole-genome bisulfite sequencing (WGBS) and reduced representation bisulfite sequencing (RRBS). MethGo provides both genomic and epigenomic analyses including: 1) coverage distribution of each cytosine;

SUBMITTER: Liao WW 

PROVIDER: S-EPMC4682368 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature