Automatic DNA Diagnosis for 1D Gel Electrophoresis Images using Bio-image Processing Technique.

ABSTRACT:

Background

DNA gel electrophoresis is a molecular biology technique for separating different sizes of DNA fragments. Applications of DNA gel electrophoresis include DNA fingerprinting (genetic diagnosis), size estimation of DNA, and DNA separation for Southern blotting. Accurate interpretation of DNA banding patterns from electrophoretic images can be laborious and error prone when a large number of bands are interrogated manually. Although many bio-imaging techniques have been proposed, none of them can fully automate the typing of DNA owing to the complexities of migration patterns typically obtained.

Results

We developed an image-processing tool that automatically calls genotypes from DNA gel electrophoresis images. The image processing workflow comprises three main steps

SUBMITTER: Intarapanich A 

PROVIDER: S-EPMC4682448 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature