Methods to Determine the Lagrangian Shear Experienced by Platelets during Thrombus Growth.

ABSTRACT: Platelets can become activated in response to changes in flow-induced shear; however, the underlying molecular mechanisms are not clearly understood. Here we present new techniques for experimentally measuring the flow-induced shear rate experienced by platelets prior to adhering to a thrombus. We examined the dynamics of blood flow around experimentally grown thrombus geometries using a novel combination of experimental (ex vivo) and numerical (in silico) methodologies. Using a microcapillary system, platelet aggregate formation was analysed at elevated shear rates in the presence of coagulation inhibitors, where thrombus formation is predominantly platelet-dependent. These approaches permit the resolution and quantification of thrombus parameters at the scale of individual platelets (2 μ

SUBMITTER: Pinar IP 

PROVIDER: S-EPMC4682839 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature