Characterization and multiplexing of 21 microsatellite markers for the herb Noccaea caerulescens (Brassicaceae).

ABSTRACT: Multiplexed microsatellite markers were developed for population genetic studies in the pseudometallophyte Noccaea caerulescens (Brassicaceae), a model species to investigate metal tolerance and hyperaccumulation in higher plants. Microsatellite loci were isolated through pyrosequencing of an enriched DNA library. Three multiplexes combining four previously published and 17 newly designed markers were developed. The new markers were screened in metallicolous and nonmetallicolous populations from southern France. The total number of alleles per locus ranged from five to 18. The observed heterozygosity per locus and per population ranged from 0 to 0.83, and expected heterozygosity ranged from 0 to 0.89. The investigated loci showed reasonable to high levels of polymorphism at the regional sc

SUBMITTER: Mousset M 

PROVIDER: S-EPMC4683039 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature