Targeting and depletion of circulating leukocytes and cancer cells by lipophilic antibody-modified erythrocytes.

ABSTRACT: There is a great interest in targeting and selective ablation of populations of circulating cells for research or therapeutic purposes. Red blood cells (RBCs) are readily available and fully biocompatible long-circulating intravascular carriers (natural life is 120days) that are amenable to chemical modifications, drug loading and reinjection. Here we demonstrate that using our previously described lipophilic ligand painting strategy, red blood cells (RBCs) could be in one step converted into targeted entities that selectively seek and bind various cells in vitro and in vivo. In vitro, RBCs modified with lipophilic anti-EpCAM or anti-CD45 antibodies efficiently bound to cancer cells and leukocytes, forming characteristic rosettes. In vivo, intravenously injected RBCs painted with anti-CD45

SUBMITTER: Mukthavaram R 

PROVIDER: S-EPMC4683104 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature