Glycolysis, but not Mitochondria, responsible for intracellular ATP distribution in cortical area of podocytes.

ABSTRACT: Differentiated podocytes, a type of renal glomerular cells, require substantial levels of energy to maintain glomerular physiology. Mitochondria and glycolysis are two major producers of ATP, but the precise roles of each in podocytes remain unknown. This study evaluated the roles of mitochondria and glycolysis in differentiated and differentiating podocytes. Mitochondria in differentiated podocytes are located in the central part of cell body while blocking mitochondria had minor effects on cell shape and migratory ability. In contrast, blocking glycolysis significantly reduced the formation of lamellipodia, a cortical area of these cells, decreased the cell migratory ability and induced the apoptosis. Consistently, the local ATP production in lamellipodia was predominantly regulated by g

SUBMITTER: Ozawa S 

PROVIDER: S-EPMC4683464 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature