SMRT Sequencing for Parallel Analysis of Multiple Targets and Accurate SNP Phasing.

ABSTRACT: Single-molecule real-time (SMRT) sequencing generates much longer reads than other widely used next-generation (next-gen) sequencing methods, but its application to whole genome/exome analysis has been limited. Here, we describe the use of SMRT sequencing coupled with barcoding to simultaneously analyze one or a small number of genomic targets derived from multiple sources. In the budding yeast system, SMRT sequencing was used to analyze strand-exchange intermediates generated during mitotic recombination and to analyze genetic changes in a forward mutation assay. The general barcoding-SMRT approach was then extended to diffuse large B-cell lymphoma primary tumors and cell lines, where detected changes agreed with prior Illumina exome sequencing. A distinct advantage afforded by SMRT seque

SUBMITTER: Guo X 

PROVIDER: S-EPMC4683651 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature