New Paenibacillus larvae bacterial isolates from honey bee colonies infected with American foulbrood disease in Egypt.

ABSTRACT: The American foulbrood disease is widely distributed all over the world and causes a serious problem for the honeybee industry. Different infected larvae were collected from different apiaries, ground in phosphate saline buffer (PSB) and bacterial isolation was carried out on nutrient agar medium. Different colonies were observed and were characterized biologically. Two bacterial isolates (SH11 and SH33) were subjected to molecular identification using 16S rRNA gene and the sequence analysis revealed that the two isolates are Paenibacillus larvae with identity not exceeding 83%. The DNA sequence alignment between the other P. larvae bacterial strains and the two identified bacterial isolates showed that all the examined bacterial strains have the same ancestor, i.e. they have

SUBMITTER: Masry SH 

PROVIDER: S-EPMC4684043 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature