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Identification of activation-tag Arabidopsis mutants with altered production of germination stimulants for Phelipanche ramosa (L.).


ABSTRACT: Germination of seeds of root parasites like broomrapes (Orobanchaceae) is tightly regulated by chemical products exuded from the roots of the host plant, known as germination stimulants (GSs). Changes in the levels of synthesis and emission of GS can allow the development of practical measures for control of the crops-harming parasitic species. However, the genes encoding enzymes responsible for GS biosynthesis are still unknown. We performed a large-scale screening of 62,000 Arabidopsis activation-tag mutants for alteration in susceptibility to Phelipanche ramosa and to identify lines with altered GS production among them. After five successive screenings we identified 36 lines with altered susceptibility to P. ramosa. Seven of them displayed altered levels of

SUBMITTER: Kirilova I 

PROVIDER: S-EPMC4684048 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature