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Single Amino Acid Switch between a Flavin-Dependent Dehalogenase and Nitroreductase.

ABSTRACT: A single mutation within a flavoprotein is capable of switching the catalytic activity of a dehalogenase into a nitroreductase. This change in function correlates with a destabilization of the one-electron-reduced flavin semiquinone that is differentially expressed in the nitro-FMN reductase superfamily during redox cycling. The diversity of function within such a superfamily therefore has the potential to arise from rapid evolution, and its members should provide a convenient basis for developing new catalysts with an altered specificity of choice.

SUBMITTER: Mukherjee A

PROVIDER: S-EPMC4684082 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature

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Single Amino Acid Switch between a Flavin-Dependent Dehalogenase and Nitroreductase.

Mukherjee Arnab A Rokita Steven E SE

Journal of the American Chemical Society 20151204 49

A single mutation within a flavoprotein is capable of switching the catalytic activity of a dehalogenase into a nitroreductase. This change in function correlates with a destabilization of the one-electron-reduced flavin semiquinone that is differentially expressed in the nitro-FMN reductase superfamily during redox cycling. The diversity of function within such a superfamily therefore has the potential to arise from rapid evolution, and its members should provide a convenient basis for developi ...[more]

PMID: 26616824

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