DNA Brushing Shoulders: Targeted Looping and Scanning of Large DNA Strands.

ABSTRACT: We present a nanofluidic device for targeted manipulations in the quarternary structure of single DNA molecules. We demonstrate the folding and unfolding of hairpin-shaped regions, similar to chromatin loops. These loops are stable for minutes at nanochannel junctions. We demonstrate continuous scanning of two DNA segments that occupy a common nanovolume. We present a model governing the stability of loop folds and discuss how the system achieves specific DNA configurations without operator intervention.

SUBMITTER: Azad Z 

PROVIDER: S-EPMC4684187 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature