The Eukaryotic Mismatch Recognition Complexes Track with the Replisome during DNA Synthesis.

ABSTRACT: During replication, mismatch repair proteins recognize and repair mispaired bases that escape the proofreading activity of DNA polymerase. In this work, we tested the model that the eukaryotic mismatch recognition complex tracks with the advancing replisome. Using yeast, we examined the dynamics during replication of the leading strand polymerase Polε using Pol2 and the eukaryotic mismatch recognition complex using Msh2, the invariant protein involved in mismatch recognition. Specifically, we synchronized cells and processed samples using chromatin immunoprecipitation combined with custom DNA tiling arrays (ChIP-chip). The Polε signal was not detectable in G1, but was observed at active origins and replicating DNA throughout S-phase. The Polε signal provided the resolution to track origin

SUBMITTER: Haye JE 

PROVIDER: S-EPMC4684283 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature