Nitrite and nitrate-dependent generation of anti-inflammatory fatty acid nitroalkenes.

ABSTRACT: A gap in our understanding of the beneficial systemic responses to dietary constituents nitrate (NO3(-)), nitrite (NO2(-)) and conjugated linoleic acid (cLA) is the identification of the downstream metabolites that mediate their actions. To examine these reactions in a clinical context, investigational drug preparations of (15)N-labeled NO3(-) and NO2(-) were orally administered to healthy humans with and without cLA. Mass spectrometry analysis of plasma and urine indicated that the nitrating species nitrogen dioxide was formed and reacted with the olefinic carbons of unsaturated fatty acids to yield the electrophilic fatty acid, nitro-cLA (NO2-cLA). These species mediate the post-translational modification (PTM) of proteins via reversible Michael addition with nucleophilic amino acids. Th

SUBMITTER: Delmastro-Greenwood M 

PROVIDER: S-EPMC4684780 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature