Deformation of a single mouse oocyte in a constricted microfluidic channel.

ABSTRACT: Single oocyte manipulation in microfluidic channels via precisely controlled flow is critical in microfluidic-based in vitro fertilization. Such systems can potentially minimize the number of transfer steps among containers for rinsing as often performed during conventional in vitro fertilization and can standardize protocols by minimizing manual handling steps. To study shape deformation of oocytes under shear flow and its subsequent impact on their spindle structure is essential for designing microfluidics for in vitro fertilization. Here, we developed a simple yet powerful approach to (i) trap a single oocyte and induce its deformation through a constricted microfluidic channel, (ii) quantify oocyte deformation in real-time using a conventional microscope, and (iii)

SUBMITTER: Luo Z 

PROVIDER: S-EPMC4684828 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature