Association of Sphingosine-1-phosphate (S1P)/S1P Receptor-1 Pathway with Cell Proliferation and Survival in Canine Hemangiosarcoma.

ABSTRACT:

Background

Sphingosine-1-phosphate (S1P) is a key biolipid signaling molecule that regulates cell growth and survival, but it has not been studied in tumors from dogs.

Hypothesis/objectives

S1P/S1P1 signaling will contribute to the progression of hemangiosarcoma (HSA).

Animals

Thirteen spontaneous HSA tissues, 9 HSA cell lines, 8 nonmalignant tissues, including 6 splenic hematomas and 2 livers with vacuolar degeneration, and 1 endothelial cell line derived from a dog with splenic hematoma were used.

Methods

This was a retrospective case series and in vitro study. Samples were obtained as part of medically necessary diagnostic procedures. Microarray, qRT-PCR, immunohistochemistry, and immunoblotting were performed to examine S1P1 expression. S1P concentrations w

SUBMITTER: Rodriguez AM 

PROVIDER: S-EPMC4684944 | biostudies-literature | 2015 Jul-Aug

REPOSITORIES: biostudies-literature