Discovering Molecules That Regulate Efferocytosis Using Primary Human Macrophages and High Content Imaging.

ABSTRACT: Defective clearance of apoptotic cells can result in sustained inflammation and subsequent autoimmunity. Macrophages, the "professional phagocyte" of the body, are responsible for efficient, non-phlogistic, apoptotic cell clearance. Controlling phagocytosis of apoptotic cells by macrophages is an attractive therapeutic opportunity to ameliorate inflammation. Using high content imaging, we have developed a system for evaluating the effects of antibody treatment on apoptotic cell uptake in primary human macrophages by comparing the Phagocytic Index (PI) for each antibody. Herein we demonstrate the feasibility of evaluating a panel of antibodies of unknown specificities obtained by immunization of mice with primary human macrophages and show that they can be distinguished based on individual

SUBMITTER: Santulli-Marotto S 

PROVIDER: S-EPMC4686065 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature