Neuronal NAD(P)H oxidases contribute to ROS production and mediate RGC death after ischemia.

ABSTRACT:

Purpose

To study the role of neuronal nicotinamide adenine dinucleotide phosphate [NAD(P)H] oxidase-dependent reactive oxygen species (ROS) production in retinal ganglion cell (RGC) death after ischemia.

Methods

Ischemic injury was induced by unilateral elevation of intraocular pressure via direct corneal cannulation. For in vitro experiments, RGCs isolated by immunopanning from retinas were exposed to oxygen and glucose deprivation (OGD). The expression levels of NAD(P)H oxidase subunits were evaluated by quantitative PCR, immunocytochemistry, and immunohistochemistry. The level of ROS generated was assayed by dihydroethidium. The NAD(P)H oxidase inhibitors were then tested to determine if inhibition of NAD(P)H oxidase altered the production of ROS within the RGCs and promo

SUBMITTER: Dvoriantchikova G 

PROVIDER: S-EPMC4686185 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature