Derivation of integration-free iPSCs from a Klinefelter syndrome patient.

ABSTRACT:

Purpose

Klinefelter syndrome (KS) (47, XXY) is the most common sex chromosome abnormality in humans. KS is characterized by gynecomastia, tall stature, small testes, low testosterone levels, learning disabilities, and behavioral problems. KS is also associated with infertility due to non-obstructive azoospermia (NOA). The mechanism underlying NOA is still poorly understood, and although there is no current treatment, the use of microdissection testicular sperm extraction (micro-TESE) followed by in vitro fertilization can result in successful conception. The generation of induced pluripotent stem (iPS) cells derived from KS patients may be useful for studying the disease mechanism and identifying novel therapies.

Methods

Cells from a KS patient were transduced with Sendai vi

SUBMITTER: Shimizu T 

PROVIDER: S-EPMC4686545 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature