3D Image-Guided Automatic Pipette Positioning for Single Cell Experiments in vivo.
Ontology highlight
ABSTRACT:
SUBMITTER: Long B
PROVIDER: S-EPMC4686883 | biostudies-literature | 2015 Dec
REPOSITORIES: biostudies-literature
Publications
3D Image-Guided Automatic Pipette Positioning for Single Cell Experiments in vivo.
Long Brian B Li Lu L Knoblich Ulf U Zeng Hongkui H Peng Hanchuan H
Scientific reports 20151222
We report a method to facilitate single cell, image-guided experiments including in vivo electrophysiology and electroporation. Our method combines 3D image data acquisition, visualization and on-line image analysis with precise control of physical probes such as electrophysiology microelectrodes in brain tissue in vivo. Adaptive pipette positioning provides a platform for future advances in automated, single cell in vivo experiments. ...[more]
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