Characterization of marine diatom-infecting virus promoters in the model diatom Phaeodactylum tricornutum.

ABSTRACT: Viruses are considered key players in phytoplankton population control in oceans. However, mechanisms that control viral gene expression in prominent microalgae such as diatoms remain largely unknown. In this study, potential promoter regions isolated from several marine diatom-infecting viruses (DIVs) were linked to the egfp reporter gene and transformed into the Pennales diatom Phaeodactylum tricornutum. We analysed their activity in cells grown under different conditions. Compared to diatom endogenous promoters, novel DIV promoter (ClP1) mediated a significantly higher degree of reporter transcription and translation. Stable expression levels were observed in transformants grown under both light and dark conditions, and high levels of expression were reported in cells in the stationary

SUBMITTER: Kadono T 

PROVIDER: S-EPMC4686930 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature