SMRT sequencing of the Campylobacter coli BfR-CA-9557 genome sequence reveals unique methylation motifs.

ABSTRACT:

Background

Campylobacter species are the most prevalent bacterial pathogen causing acute enteritis worldwide. In contrast to Campylobacter jejuni, about 5 % of Campylobacter coli strains exhibit susceptibility to restriction endonuclease digestion by DpnI cutting specifically 5'-G(m)ATC-3' motifs. This indicates significant differences in DNA methylation between both microbial species. The goal of the study was to analyze the methylome of a C. coli strain susceptible to DpnI digestion, to identify its methylation motifs and restriction modification systems (RM-systems), and compare them to related organisms like C. jejuni and Helicobacter pylori.

Results

Using one SMRT cell and the PacBio RS sequencing technology followed by PacBio Modification and Motif Analysis the complet

SUBMITTER: Zautner AE 

PROVIDER: S-EPMC4687069 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature