De novo transcriptome sequencing and analysis of Coccinella septempunctata L. in non-diapause, diapause and diapause-terminated states to identify diapause-associated genes.

ABSTRACT:

Background

The most common ladybird beetle, Coccinella septempunctata L., is an excellent predator of crop pests such as aphids and white flies, and it shows a wide range of adaptability, a large appetite and a high reproductive ability. Diapause research plays an important role in the artificial propagation and shelf-life extension of insect products. Although this lady beetle's regulatory, physiological and biochemical characteristics in the diapause period are well understood, the molecular mechanism of diapause remains unknown. Therefore, we collected female adults in three different states, i.e., non-diapause, diapause and diapause termination, for transcriptome sequencing.

Results

After transcriptome sequencing using the Illumina HiSeq 2500 platform with pretreatment,

SUBMITTER: Qi X 

PROVIDER: S-EPMC4687109 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature